Accuri C6 Flow Cytometer Manual

Download Accuri C6 Flow Cytometer Manual

Accuri c6 flow cytometer manual download free. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. This manual provides information on cytometer requirements and specifications, setup, and maintenance.

It also includes common troubleshooting help and service information. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. It also includes common troubleshooting help and service information.

BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed.

BD Accuri C6 Software will display this notification based on whichever trigger is reached first. Page Decontamination Fluid Cycle 6. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Turn on the computer and log in with your UCF info. Start the Accuri CSampler software. Press the Power button on the BD Accuri C6 instrument face panel.

The instrument will begin a 5 File Size: KB. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation.

Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O ( µm filter), or equivalent, when the cytometer is not running samples. BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. If you have questions or require further clarification, see the BD Accuri C6 Flow Cytometer Instrument Manual or contact Accuri Technical Support. If you are outside the US or EU, please contact your local BD support.

Samples were collected on the BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software. CD4 + lymphocytes were identified and gated by light scatter profile and fluorescence (data not shown).

Results: A. A CD25 vs CD plot was used to identify CD25 bright CD dim Tregs and non-Treg CD4 + cells. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. Take a leap in flow cytometry with the BD Accuri™ C6 Plus.

This portable and compact system holds the analytical power of a cytometer twice its size. Cited in over 9, publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. Instructional video on the basics of using BYU's Accuri C6 cytometer.

Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of File Size: KB.

Manuals and User Guides for BD Accuri C6. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open) Changing the Workstation Computer Settings for Windows ® 7.

Figure In-Line Sheath Filter. Figure Interference Filter. Figure Fluidics Harness. Figure Proper. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of Decem. Service for BD Accuri C6 systems and their related options will continue until Ap.

All current service contracts will be honored. Current extended warranties will not be affected and will be supported until they expire. One-year and multi-year extended warranty renewals will continue to be. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. I would say a must-have equipment for every lab foc. The cytometer will stop automatically when the time limit is reached.

9) Leave the tube on the SIP until the cytometer is used again. It is recommended that the system be shut down if it will not be run again that day. Use only approved fluids, described in the Approved Reagents table of the BD Accuri C6 Flow Cytometer Instrument Manual. Accuri C6 Flow Cytometer Instrument Manual Accuri C6 Software User’s Guide, Chapter 1, The Basics for a complete description of how to run and analyze bead results.

Compare the new data to that provided with the C6 and email the file created in Page 4/ Get Free Accuri C6 User Guide step 20 to [email protected] cawn.xn----7sbbrk9aejomh.xn--p1ai *Steps may be skipped if you purchased your computer from.

The BD Accrui C6 Cytometer -The cytometer has 2 lasers: Blue nm laser (3 colors) -Red (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you Filter Settings And possible fluorochrome: FL1 /30nm (FITC/GFP) FL2 /40nm.

2 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER Running Validation Beads (Daily Start-Up) Saving Validation Bead Data Analyzing and Recording Validation Bead Data Monitoring Validation Bead Data Troubleshooting Validation 3 DATA ACQUISITION Viewing the Collect Tab Collecting. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one).

Accuri C6 is a digital PC based bench top analyser, 2 laser system. Standard fluorochrome in use are identical to FACS Calibur. Even more colors to choose from with selectable lasers and filter replacement. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research.

As a result, flow cytometry has become a. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (nm) and a Red (nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC.

BD Accuri C6 flow cytometer for the analysis of plant genome sizes. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of magnitude (a range of to pg).

This covers most of the C-values encountered in the flowering plants found across. BD Accuri C6 with plate handler April This protocol contains instructions for setting up an Accuri C6 with plate handler. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at [email protected] Size: 1MB.

It's free to register here toget Bd Accuri C6 Flow Cytometer Manual Free Books Book file PDF. file Bd Accuri C6 Flow Cytometer Manual Free Books Book Free Download PDF at Our eBook Library. This Book have some digitalformats such us: kindle, epub, ebook, paperbook, and another formats. Here is The Complete PDF Library BD Accuri™ C6 Flow Cytometer Instrument Manual Controlling The. David (and all), As regards the pumps on the Accuri C6 it is my understanding that the "pump servicing" you refer to is just a tubing change on the two peristaltic pumps (sheath & waste).

It is all luer-lock fittings and shouldn't take any longer than minutes. This is something that is done "on-site" by the user and does not require a service technician to do, nor does it require you to.

Unless otherwise noted, BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © BD. To improve BD products and services and. 4 MANUAL DATA ACQUISITION . 24 Viewing the Manual Collect Tab . 24 Collecting Sample Data . 27 Setting the Fluidics Rate .

28 Setting the Threshold . 28 Assigning a Plate Type . 30 Naming the Plate . 31 Naming the Sample . 32 Setting a Run Limit . 33 Running the Sample . 34 Washing the SIP Between. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER Running Validation Beads (Daily Start-Up) Saving Validation Bead Data Analyzing and Recording Validation Bead Data Monitoring Validation Bead Data Troubleshooting Validation 4 MANUAL DATA ACQUISITION Viewing the Manual Collect Tab. Easy to use, simple to maintain, and affordable, the BD Accuri™ C6 Plus personal flow cytometer brings cell analysis within reach for new applications and new flow cytometry users.

Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a. BD Accuri C6 Plus flow cytometer With its compact 11 x x in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications.

Accuri C6 Flow Cytometer Instrument Manual The Accuri C6 Flow Cytometer does not produce aerosols during normal operation Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O ( µm filter), or equivalent, when the cytometer is not running samples Accuri C6 User Guide - cawn.xn----7sbbrk9aejomh.xn--p1ai Download Ebook Accuri C6 User Guide.

A Guide to Absolute Counting on the Accuri C6 Flow Cytometer Page 2 of 4 Figure 3. Discriminating cell clumps using a plot of FSC-A versus FSC-H. a) P2 is the single cell gate, where increasing FSC-A channel correlates linearly with increasing FSC-H signal. The P3 gate is cell clumps. b and c) Serial dilutions of the primary sample shown in 3a. The percentage of cell clumps (P3) decreases with. BD Accuri™ personal flow cytometers bring immunology and stem cell studies to the benchtop.

With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. Two additional features further streamline the process. Accuri C6 Flow Cytometry Analyzer The Satelite Facility at Burrill Hall (Dr. David Kranz's lab) (click link for campus map) is equipped with an Accuri C6 4-color flow cytometry analyzer.

After receiving instrument training from the Flow Cytometry Facility staff, users are granted 24/7 access to the lab (advanced booking is required). At my lab, we have an Accuri C6 cytometer. We had a problem with its valves and it was solved in last November. Recently, however, we've noticed there's a red light always on inside of the device.

Flow cytometry eliminates manual counting errors and allows simultaneous quantitative assessment of multiple cellular parameters at the single-cell level within minutes of water sampling. The BD Accuri C6, the first personal flow cytometer, is a promising candidate for monitoring drinking water because of its portability, open fluidics system, and ability to determine sample volume and. However, instruments like the Beckman Coulter Gallios, Life Technologies Attune, BD Accuri C6, and MoFlo Astrios will scale data from 6 to 10 decades.

Since FlowJo must scale the data identically to what you see on the machine, we have implemented a great new feature in v10 of FlowJo that will create a specific preference set based on the cytometer on which the data was acquired when loaded.

BD Accuri C6 flow cytometer analysis. All fluorescence signals of labeled spermatozoa were analyzed by the BD Accuri C6 Flow Cytometer and C6 Plus Flow Cytometer (Becton Dickinson, San Jose, CA, USA). A total of 10 spermatozoa was counted with fluidic set at “slow” with flow rate of Location: Rockville Pike, Bethesda, MD.

BD Accuri™ flow cytometers. 8 February The Cytohub. email. Print. Linkedin. Facebook. 0. Twitter. 0. Why small means big. The BD Accuri™ is a personal bench-top flow cytometer designed to make flow-cytometry easier and more accessible. But are you aware that this small machine has been doing very big things? A Google Scholar search reveals more than citations associated with BD.

The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The BD Accuri C6 contains the following co-linear lasers and emission filters which can detect up to 4 fluorescent parameters and 2 light scatter parameters: nm (Blue. Our Accuri Gen 1 had to be replaced as BD can now longer service it.

We had no redundancy in the lab for flow cytometry. Lori McDonald of Certified GeneTool Inc. found us a replacement Accri Gen 2 and provided QC bead tests after it’s last PM, and before shipping so that we could compare with our installation. She accomplished all this in less than a week and arranged for 2day delivery.

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